McCutchen B F, Choudary P V, Crenshaw R, Maddox D, Kamita S G, Palekar N, Volrath S, Fowler E, Hammock B D, Maeda S
Department of Entomology, University of California, Davis 95616.
Biotechnology (N Y). 1991 Sep;9(9):848-52. doi: 10.1038/nbt0991-848.
Recombinant nuclear polyhedrosis viruses (NPVs) expressing insect-selective toxins, hormones, or enzymes could enhance their insecticidal properties. We have constructed a recombinant, polyhedrin-positive Autographa californica NPV (AcNPV) that is orally infectious and expresses an insect-selective toxin (AaIT), isolated from the scorpion Androctonus australis, under the control of the p10 promoter. Bioassays with the recombinant baculovirus on 2nd instar larvae of Heliothis virescens demonstrated a significant decrease in the time to kill (LT50 88.0 hours) compared to wild-type AcNPV (LT50 125 hours). Production of AaIT was confirmed by western blot analysis of larval hemolymph from infected H. virescens, and bioassays with larvae of Sarcophaga falculata.
表达昆虫选择性毒素、激素或酶的重组核型多角体病毒（NPV）可增强其杀虫特性。我们构建了一种重组的、多角体蛋白阳性的苜蓿银纹夜蛾核型多角体病毒（AcNPV），它具有口服感染性，并在p10启动子的控制下表达一种从蝎子澳毒蛛分离出的昆虫选择性毒素（AaIT）。用重组杆状病毒对棉铃虫二龄幼虫进行的生物测定表明，与野生型AcNPV（LT50为125小时）相比，致死时间（LT50为88.0小时）显著缩短。通过对感染棉铃虫幼虫血淋巴的蛋白质免疫印迹分析以及对肥须亚麻蝇幼虫的生物测定，证实了AaIT的产生。
