Kimura Y, Asada Y, Oka T, Kuwahara M
Department of Bioresource Science, Faculty of Agriculture, Kagawa University, Japan.
Appl Microbiol Biotechnol. 1991 Jul;35(4):510-4. doi: 10.1007/BF00169758.
A cDNA clone, lambda LPO-1, encoding a major lignin peroxidase from the basidiomycete Bjerkandera adusta was isolated and characterized. The nucleotide sequence of lambda LPO-1 predicts a mature protein consisting of 349 amino acids with a molecular weight of 37,225 preceded by a signal peptide of 23 amino acid residues. We have also cloned and sequenced the gene encoding lignin peroxidase from B. adusta. Comparison of these sequences reveals a lignin peroxidase gene structure consisting of 1,116 bp of protein-encoding DNA that is interrupted by four intervening sequences. The putative eukaryotic regulatory sequence, a TATA box, is present at position -75 relative to the translational initiation codon. Amino acid sequence homology between the coding regions of lambda LPO-1 and of the lignin peroxidase cDNA clone lambda ML-1 from Phanerochaete chrysosporium is 61%.
分离并鉴定了一个来自担子菌烟管菌的编码主要木质素过氧化物酶的cDNA克隆λLPO-1。λLPO-1的核苷酸序列预测成熟蛋白由349个氨基酸组成，分子量为37225，前面有一个23个氨基酸残基的信号肽。我们还克隆并测序了烟管菌中编码木质素过氧化物酶的基因。这些序列的比较揭示了一个木质素过氧化物酶基因结构，其由1116bp的蛋白质编码DNA组成，该DNA被四个间隔序列中断。假定的真核调控序列，即TATA框，位于相对于翻译起始密码子的-75位置。λLPO-1的编码区与来自黄孢原毛平革菌的木质素过氧化物酶cDNA克隆λML-1的编码区之间的氨基酸序列同源性为61%。
