Huoponen K, Ollikka P, Kälin M, Walther I, Mäntsälä P, Reiser J
Department of Biochemistry, University of Turku, Finland.
Gene. 1990 Apr 30;89(1):145-50. doi: 10.1016/0378-1119(90)90218-g.
Two closely linked lignin peroxidase (LPO)-encoding genes (lpo) from Phanerochaete chrysosporium were isolated. Nucleotide sequence studies indicated that the two genes are separated by 1.3 kb of flanking DNA and transcribed in opposite directions. Cloned P. chrysosporium lpo gene probes have been shown to hybridize to multiple sequences present in the DNAs of the white-rot fungi, Bjerkandera adusta, Coriolus versicolor and Fomes lignosus, but no hybridization was detected with DNA from Pleurotus ostreatus. Thus, lpo gene families appear to be common in a number of lignin-degrading basidiomycetes, some of which have not yet been shown to produce LPO proteins.
从黄孢原毛平革菌中分离出了两个紧密相连的编码木质素过氧化物酶（LPO）的基因（lpo）。核苷酸序列研究表明，这两个基因被1.3 kb的侧翼DNA隔开，并以相反的方向转录。已证明克隆的黄孢原毛平革菌lpo基因探针可与白腐真菌烟管菌、云芝和木蹄层孔菌DNA中的多个序列杂交，但未检测到与糙皮侧耳DNA的杂交。因此，lpo基因家族似乎在许多木质素降解担子菌中很常见，其中一些尚未被证明能产生LPO蛋白。
