粪肠球菌的固定化及处理以实现精氨酸持续转化为瓜氨酸。

Immobilization and treatment of Streptococcus faecalis for the continuous conversion of arginine into citrulline.

作者信息

Cottenceau G, Dherbomez M, Lubochinsky B, Lettellier F

机构信息

Laboratoire de Biochimie Cellulaire, Institut Universitaire de Technologie, La Rochelle, France.

出版信息

Enzyme Microb Technol. 1990 May;12(5):355-60. doi: 10.1016/0141-0229(90)90164-l.

DOI:10.1016/0141-0229(90)90164-l

PMID:1367441

Abstract

Citrulline is one of the steps of the arginine dihydrolase system of Streptococcus faecalis. We have shown that the bacteria, immobilized in polyacrylamide gel and treated with Cetyl trimethyl ammonium bromide (CTAB) or heat, were able to convert arginine to citrulline. Used continuously in a column reactor, the entrapped cells have a stable enzymatic activity for at least 30 days at 45 degrees C.

摘要

瓜氨酸是粪链球菌精氨酸二水解酶系统的步骤之一。我们已经表明，固定在聚丙烯酰胺凝胶中并用十六烷基三甲基溴化铵（CTAB）或加热处理的细菌能够将精氨酸转化为瓜氨酸。在柱式反应器中连续使用时，截留的细胞在45℃下具有至少30天的稳定酶活性。