Border P M, Howard J J, Plastow G S, Siggens K W
Group Research Laboratory, Cambridge, UK.
Lett Appl Microbiol. 1990 Sep;11(3):158-62. doi: 10.1111/j.1472-765x.1990.tb00149.x.
Five oligonucleotide sequences are described that were used as primers in the polymerase chain reaction (PCR) to amplify specific sequences from Listeria DNA. When all five primers were used in combination, three PCR products were possible; a Listeria specific product that occurs with DNA from any Listeria sp., a Listeria monocytogenes specific product that occurs only in the presence of DNA from this organism and a universal product that is found using DNA from any bacterial source. The occurrence of these PCR products was used as a diagnostic test on bacteria isolated from various food samples to detect Listeria sp. and L. monocytogenes.
描述了五条寡核苷酸序列，它们在聚合酶链反应（PCR）中用作引物，以从李斯特菌DNA中扩增特定序列。当将所有五条引物组合使用时，可能会产生三种PCR产物；一种李斯特菌特异性产物，可与任何李斯特菌属的DNA产生；一种单核细胞增生李斯特菌特异性产物，仅在存在该生物体的DNA时出现；以及一种通用产物，可使用来自任何细菌来源的DNA找到。这些PCR产物的出现被用作对从各种食品样本中分离出的细菌进行诊断测试，以检测李斯特菌属和单核细胞增生李斯特菌。
