Lawrence L M, Gilmour A
Department of Food Science (Food Microbiology), Queen's University of Belfast, Northern Ireland, United Kingdom.
Appl Environ Microbiol. 1994 Dec;60(12):4600-4. doi: 10.1128/aem.60.12.4600-4604.1994.
The incidence of Listeria spp. and Listeria monocytogenes in a poultry processing plant and in raw and cooked poultry products was determined over a 6-month period. Within the raw and cooked poultry processing environments, 46% (36 of 79) and 29% (51 of 173) of the samples contained Listeria spp. while 26% (21 of 79) and 15% (27 of 173) contained L. monocytogenes, respectively. Various sites within the processing environment were found to be consistently positive for L. monocytogenes throughout the entire sampling period. Of the raw and cooked products tested, 91% (53 of 58) and 8% (8 of 96) were found to contain Listeria spp. while 59% (34 of 58) and 0% (0 of 96) contained L. monocytogenes, respectively. Although L. monocytogenes was not detected in the cooked products examined, the presence of other Listeria spp. highlights the potential which exists for postprocessing contamination. Multiplex PCR proved to be a convenient and time-saving technique for rapid confirmation of Listeria spp. and L. monocytogenes in a single reaction.
在6个月的时间里，对一家家禽加工厂以及生熟禽肉产品中的李斯特菌属和单核细胞增生李斯特菌的发生率进行了测定。在生熟禽肉加工环境中，分别有46%（79份样本中的36份）和29%（173份样本中的51份）的样本含有李斯特菌属，而含有单核细胞增生李斯特菌的样本分别为26%（79份中的21份）和15%（173份中的27份）。在整个采样期间，加工环境中的各个地点被发现单核细胞增生李斯特菌一直呈阳性。在所检测的生熟产品中，分别有91%（58份中的53份）和8%（96份中的8份）含有李斯特菌属，而含有单核细胞增生李斯特菌的分别为59%（58份中的34份）和0%（96份中的0份）。尽管在所检测的熟制品中未检测到单核细胞增生李斯特菌，但其他李斯特菌属的存在凸显了加工后污染存在的可能性。多重PCR被证明是一种方便且省时的技术，可在单一反应中快速确认李斯特菌属和单核细胞增生李斯特菌。
