来自乳酸乳球菌乳脂亚种质粒pDI - 21的乳糖磷酸转移酶（Lac - PTS）和塔格糖1,6 - 二磷酸醛缩酶基因的体外表达
In vitro expression of Lac-PTS and tagatose 1,6-bisphosphate aldolase genes from Lactococcus lactis subsp. cremoris plasmid pDI-21.
作者信息
Yu P L, Hodge R A, Li X P
机构信息
Department of Biotechnology, Massey University, Palmerston North, New Zealand.
出版信息
Appl Microbiol Biotechnol. 1990 Sep;33(6):677-9. doi: 10.1007/BF00604936.
A 4.4-kb EcoR1-EcoR1 DNA fragment from the Lactococcus lactis subsp. cremoris plasmid pDI-21 encoded the tagatose 1,6-bisphosphate (TBP) aldolase gene and the Lac-PTS genes. In vitro transcription-translation using Escherichia coli S30 extract showed the synthesis of 41,000-, 23,000- and 12,000-dalton proteins which correspond to the TBP-aldolase, Lac-PTS enzyme II, and factor III proteins respectively.
来自乳酸乳球菌亚种cremoris质粒pDI - 21的一个4.4千碱基对的EcoR1 - EcoR1 DNA片段编码了塔格糖1,6 - 二磷酸（TBP）醛缩酶基因和乳糖磷酸转移酶（Lac - PTS）基因。使用大肠杆菌S30提取物进行的体外转录 - 翻译实验表明，分别合成了分子量为41,000、23,000和12,000道尔顿的蛋白质，它们分别对应于TBP - 醛缩酶、Lac - PTS酶II和因子III蛋白。
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