Hori N, Watanabe M, Sunagawa K, Uehara K, Mikami Y
Tobacco Science Research Laboratory, Japan Tobacco Inc., Yokohama.
J Biotechnol. 1991 Feb;17(2):121-31. doi: 10.1016/0168-1656(91)90003-e.
5-Methyluridine was produced continuously from thymine and inosine by immobilized enzymes, which consisted of thermostable purine nucleoside phosphorylase and thermostable pyrimidine nucleoside phosphorylase obtained from Bacillus stearothermophilus JTS 859. The process was carried out in a column reactor at 60 degrees C for 17 d without any bacterial contamination under non-aseptical conditions. Half-lives of the activity of the immobilized enzymes were 47 d and 4.5 d at 60 degrees C and 70 degrees C, respectively, although half-life of the crude enzyme was only 14 h at 70 degrees C.
通过固定化酶由胸腺嘧啶和肌苷连续生产5-甲基尿苷，该固定化酶由嗜热脂肪芽孢杆菌JTS 859中获得的耐热嘌呤核苷磷酸化酶和耐热嘧啶核苷磷酸化酶组成。该过程在柱式反应器中于60℃下进行17天，在非无菌条件下无任何细菌污染。固定化酶活性在60℃和70℃下的半衰期分别为47天和4.5天，而粗酶在70℃下的半衰期仅为14小时。
