State Key Laboratory of Chemical Resources Engineering, Beijing University of Chemical Technology, Beijing 100029, People's Republic of China.
Bioorg Med Chem Lett. 2012 Mar 1;22(5):2102-4. doi: 10.1016/j.bmcl.2011.12.142. Epub 2012 Jan 10.
5-Methyluridine has been synthesized in high yield using guanosine and thymine as starting materials in the presence of highly thermostable recombinant purine nucleoside phosphorylase (PNP) and uridine phosphorylase (UP) obtained from hyperthermophilic aerobic crenarchaeon Aeropyrum pernix. Key reaction parameters such as pH, temperature, concentration of buffer and substrates were investigated. At the optimal conditions, 5-methyluridine was achieved in yield 85% with a guanosine conversion of 96% in 10ml scale. The process can be performed at high temperature, which will highly increase the solubility of substrates, therefore, this process is suitable for the industry application.
5-甲基尿苷已在高温好氧泉古菌 Aeropyrum pernix 来源的高度热稳定重组嘌呤核苷磷酸化酶 (PNP) 和尿苷磷酸化酶 (UP) 的存在下，使用鸟苷和胸腺嘧啶作为起始原料高产合成。研究了关键反应参数，如 pH 值、温度、缓冲液和底物浓度。在最佳条件下，10ml 规模下，5-甲基尿苷的产率为 85%，鸟苷转化率为 96%。该过程可以在高温下进行，这将极大地提高底物的溶解度，因此，该过程适用于工业应用。
