Hopf N W, Yonsel S, Deckwer W D
Biochemical Engineering Division, GBF-National Research Institute for Biotechnology, Braunschweig, Federal Republic of Germany.
Appl Microbiol Biotechnol. 1990 Dec;34(3):350-3. doi: 10.1007/BF00170057.
Using the method of equi-inocular synchronized comparative fermentation (EISCF) the cultivation of Sorangium cellulosum So ce 10 and production of the polyketide antibiotic ambruticin S was compared in stirred-tank and air-lift reactors of different geometry. This method requires that inocula originate from the same pre-culture and cultivation parameters are synchronized to similar values. Similar ambruticin yields were obtained from both reactor systems provided that the concentration of dissolved oxygen (DO) was maintained above a certain value (ca. 40%). For cultivation of S. cellulosum it is the DO level rather than the oxygen transfer rate which presents the proper criterion for scale-up and comparative reactor studies.
采用等目同步比较发酵（EISCF）方法，在不同几何形状的搅拌罐式反应器和气升式反应器中，对纤维堆囊菌So ce 10的培养及聚酮类抗生素安布罗霉素S的生产进行了比较。该方法要求接种物源自同一预培养物，且培养参数同步至相似值。只要溶解氧（DO）浓度维持在某一特定值（约40%）以上，两种反应器系统均可获得相似的安布罗霉素产量。对于纤维堆囊菌的培养而言，决定放大培养及比较反应器研究的合适标准是溶解氧水平而非氧传递速率。
