Hopf N W, Yonsel S, Deckwer W D
Biochemical Engineering Division, GB, Gesellschaft für Biotechnologische Forschung mbH, Braunschweig, Federal Republic of Germany.
Appl Microbiol Biotechnol. 1990 Feb;32(5):499-504. doi: 10.1007/BF00173717.
The antimycoticum ambruticin was produced by cultivating the myxobacterium Sorangium cellulosum in shake flasks and stirred tank reactors with 10- and 30-l working volumes. During growth and product formation amino acid concentrations in the complex media were measured by HPLC. In the logarithmic growth phase free aspartate and glutamate were assimilated without product formation. After consumption of aspartate and glutamate, growth was limited and ambruticin production set in. The effects of biological parameters such as age, amount of inoculum, pretreatment of cells before inoculation and time of precultivation were also investigated.
抗真菌剂安布罗霉素是通过在工作体积为10升和30升的摇瓶和搅拌罐反应器中培养粘细菌纤维堆囊菌生产的。在生长和产物形成过程中，通过高效液相色谱法测定复合培养基中的氨基酸浓度。在对数生长期，游离天冬氨酸和谷氨酸被同化而不形成产物。天冬氨酸和谷氨酸消耗完后，生长受到限制，安布罗霉素开始产生。还研究了诸如菌龄、接种量、接种前细胞预处理和预培养时间等生物学参数的影响。
