使用流动注射分析（FIA）在线测定重组大肠杆菌中的细胞内β-半乳糖苷酶活性。

On-line determination of intracellular beta-galactosidase activity in recombinant Escherichia coli using flow injection analysis (FIA).

作者信息

Kracke-Helm H A, Brandes L, Hitzmann B, Rinas U, Schügerl K

机构信息

Institut für Technische Chemie, Universität Hannover, F.R.G.

出版信息

J Biotechnol. 1991 Aug;20(1):95-104. doi: 10.1016/0168-1656(91)90038-w.

DOI:10.1016/0168-1656(91)90038-w

PMID:1367539

Abstract

A flow injection analysis (FIA) system was developed for the determination of cytoplasmic beta-galactosidase activity in recombinant Escherichia coli. The FIA system and its application for on-line monitoring of beta-galactosidase production during cultivation of recombinant E. coli in a 60-l airlift tower loop reactor is described. The results demonstrate that an FIA assay in conjunction with a cell disintegration step can be applied successfully for on-line monitoring of intracellular protein formation.

摘要

开发了一种流动注射分析（FIA）系统，用于测定重组大肠杆菌中的细胞质β-半乳糖苷酶活性。描述了该FIA系统及其在60升气升塔式环流反应器中培养重组大肠杆菌期间在线监测β-半乳糖苷酶产生的应用。结果表明，结合细胞裂解步骤的FIA测定可成功应用于细胞内蛋白质形成的在线监测。