A chemiluminescent assay for quantitation of beta-galactosidase in the femtogram range: application to quantitation of beta-galactosidase in lacZ-transfected cells.
作者信息
Jain V K, Magrath I T
机构信息
Pediatric Branch, National Cancer Institute, National Institutes of Health, Bethesda, Maryland 20892.
出版信息
Anal Biochem. 1991 Nov 15;199(1):119-24. doi: 10.1016/0003-2697(91)90278-2.
PMID:1807155
Abstract
An optimized chemiluminescent assay for beta-galactosidase using a chemiluminescent substrate AMPGD (3-(4-methoxyspiro[1,2-dioxetane-3,2'-tricyclo-[3.3.1. 1(3,7)]decan]-4- yl)phenyl-beta-D-galactopyranoside) is described. This assay is rapid and sensitive and can detect as little as 2 fg of beta-galactosidase. Its use for the quantitation of beta-galactosidase in cells transfected with lacZ-expressing vectors is described. It is possible to detect a single cell stably expressing lacZ by this technique.
摘要
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