磷-32在大肠杆菌核酸合成微量测定中的应用。
Use of phosphorus-32 in microassay for nucleic acid synthesis in Escherichia coli.
作者信息
HANAWALT P
出版信息
Science. 1959 Aug 14;130(3372):386-7. doi: 10.1126/science.130.3372.386.
PMID:13675760
Abstract
A method was developed to facilitate studies of ribonucleic acid and deoxyribonucleic acid synthesis in growing bacterial cultures of small volume. The nucleic acid fractions were separated by means of a modified Schmidt-Thannhauser procedure applied to micro quantities of P(32)-labeled cells collected on membrane filters. The validity and usefulness of the method are discussed.
摘要
已开发出一种方法，以促进对小体积生长细菌培养物中核糖核酸和脱氧核糖核酸合成的研究。核酸组分通过改良的施密特 - 坦豪泽程序进行分离，该程序应用于收集在膜滤器上的微量P(32)标记细胞。讨论了该方法的有效性和实用性。
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