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乙醇对大肠杆菌细胞的影响。乙醇处理导致DNA合成增强。

Effect of ethanol on Escherichia coli cells. Enhancement of DNA synthesis due to ethanol treatment.

作者信息

Basu T, Poddar R K

机构信息

Department of Biophysics, Molecular Biology and Genetics, University College of Science, Calcutta, India.

出版信息

Folia Microbiol (Praha). 1994;39(1):3-6. doi: 10.1007/BF02814520.

DOI:10.1007/BF02814520
PMID:8181778
Abstract

Increased ethanol concentration in the nutrient medium gradually slowed down the growth of Escherichia coli cells. However, during growth in the presence of 5% ethanol, DNA synthesis per cell increased about 2.5-fold compared to control cells. There was a 40-45% increase in plasmid copy number in the ethanol-treated cells.

摘要

营养培养基中乙醇浓度的增加逐渐减缓了大肠杆菌细胞的生长。然而，在5%乙醇存在的情况下生长时，与对照细胞相比，每个细胞的DNA合成增加了约2.5倍。乙醇处理的细胞中质粒拷贝数增加了40 - 45%。

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本文引用的文献

1
Effect of continued exposition to ethanol on activity of the ammonium and fructose transport systems in Saccharomyces cerevisiae var. ellipsoideus.持续暴露于乙醇对椭圆酿酒酵母氨和果糖运输系统活性的影响。
Biotechnol Bioeng. 1991 Feb 20;37(4):389-91. doi: 10.1002/bit.260370415.
2
Effects of ethanol and other alkanols on the general amino acid permease of Saccharomyces cerevisiae.乙醇和其他链烷醇对酿酒酵母通用氨基酸通透酶的影响。
Biotechnol Bioeng. 1984 Apr;26(4):403-5. doi: 10.1002/bit.260260422.
3
Use of phosphorus-32 in microassay for nucleic acid synthesis in Escherichia coli.
磷-32在大肠杆菌核酸合成微量测定中的应用。
Science. 1959 Aug 14;130(3372):386-7. doi: 10.1126/science.130.3372.386.
4
Ethanol-induced changes in lipid composition of Escherichia coli: inhibition of saturated fatty acid synthesis in vitro.乙醇诱导的大肠杆菌脂质组成变化：体外对饱和脂肪酸合成的抑制
Arch Biochem Biophys. 1980 Sep;203(2):565-71. doi: 10.1016/0003-9861(80)90213-1.
5
Effects of ethanol on the Escherichia coli plasma membrane.乙醇对大肠杆菌质膜的影响。
J Bacteriol. 1984 Jan;157(1):233-9. doi: 10.1128/jb.157.1.233-239.1984.
6
Calcium-dependent bacteriophage DNA infection.钙依赖性噬菌体DNA感染。
J Mol Biol. 1970 Oct 14;53(1):159-62. doi: 10.1016/0022-2836(70)90051-3.
7
Deoxyribonucleic acid synthesis in cell-free extracts. IV. Purification and catalytic properties of deoxyribonucleic acid polymerase III.无细胞提取物中的脱氧核糖核酸合成。IV. 脱氧核糖核酸聚合酶III的纯化及催化特性
J Biol Chem. 1972 Sep 10;247(17):5369-75.
8
Ethanol-induced genotoxicity.
Mutat Res. 1985 May-Jun;143(1-2):23-7. doi: 10.1016/0165-7992(85)90099-5.
9
The replicative origin of the E. coli chromosome binds to cell membranes only when hemimethylated.大肠杆菌染色体的复制起点仅在半甲基化时与细胞膜结合。
Cell. 1988 Jul 1;54(1):127-35. doi: 10.1016/0092-8674(88)90186-9.
10
Effects of antibiotics and other inhibitors on ATP-dependent protein translocation into membrane vesicles.抗生素及其他抑制剂对ATP依赖的蛋白质转运至膜囊泡过程的影响。
J Bacteriol. 1987 Jun;169(6):2373-9. doi: 10.1128/jb.169.6.2373-2379.1987.