Ohler Leopold, Geissler Klaus, Hinterberger Wolfgang
Division of Hematology, Department of Internal Medicine I, University of Vienna, Vienna, Austria.
Wien Klin Wochenschr. 2003 Aug 14;115(13-14):537-46. doi: 10.1007/BF03041036.
Hematopoietic progenitor cells are capable of forming colonies of mature blood cells in semisolid media in response to specific growth factors. Colony assays have been extensively used for many years to study normal and malignant hematopoiesis in vitro. In fact, these assays have provided an excellent research tool for investigating growth and differentiation of progenitor cells in response to positive and negative regulators of hematopoiesis. However, apart from their role in basic research, colony assays are also widely used in routine clinical practice in the diagnosis of various hematologic disorders, such as aplastic anemia, myelodysplastic syndromes and myeloproliferative disorders. This review summarizes our current knowledge on the diagnostic value and prognostic significance of the growth of progenitor cells in peripheral blood and bone marrow in patients with myeloid malignancies.
造血祖细胞能够在半固体培养基中响应特定生长因子形成成熟血细胞集落。多年来，集落分析已被广泛用于体外研究正常和恶性造血。事实上，这些分析为研究祖细胞在造血正负调节因子作用下的生长和分化提供了出色的研究工具。然而，除了在基础研究中的作用外，集落分析在常规临床实践中也广泛用于诊断各种血液系统疾病，如再生障碍性贫血、骨髓增生异常综合征和骨髓增殖性疾病。本综述总结了我们目前对髓系恶性肿瘤患者外周血和骨髓中祖细胞生长的诊断价值和预后意义的认识。
