Swoger Jim, Huisken Jan, Stelzer Ernst H K
Cell Biology and Biophysics Programme, European Molecular Biology Laboratory, Meyerhofstrasse 1, D-69117 Heidelberg, Germany.
Opt Lett. 2003 Sep 15;28(18):1654-6. doi: 10.1364/ol.28.001654.
The multiple imaging axis microscope (MIAM) is a wide-field optical microscope that observes a sample simultaneously from multiple directions without requiring the sample to be rotated or tilted. The prototype is capable of high-resolution imaging of the interior of a 300-microm-diameter sample consisting of fluorescent microbeads suspended in an agarose gel. Compared with a single-axis system, the MIAM can achieve a reduction of the axial point-spread function elongation by a factor of 5.8 and a 3.5-fold improvement in volume resolution by simple linear image combination techniques.
多成像轴显微镜（MIAM）是一种宽视场光学显微镜，它可以从多个方向同时观察样本，而无需旋转或倾斜样本。该原型能够对直径为300微米的样本内部进行高分辨率成像，该样本由悬浮在琼脂糖凝胶中的荧光微珠组成。与单轴系统相比，通过简单的线性图像组合技术，MIAM可以将轴向点扩散函数的伸长减少5.8倍，并将体积分辨率提高3.5倍。
