光学重聚焦三维宽场荧光寿命成像显微镜

Optical refocusing three-dimensional wide-field fluorescence lifetime imaging microscopy.

作者信息

Wu Qiang, Guo Shangyu, Ma Yinxing, Gao Feng, Yang Chengliang, Yang Ming, Yu Xuanyi, Zhang Xinzheng, Rupp Romano A, Xu Jingjun

机构信息

The MOE Key Laboratory of Weak Light Nonlinear Photonics, TEDA Applied Physics School and School of Physics, Nankai University, Tianjin, China.

出版信息

Opt Express. 2012 Jan 16;20(2):960-5. doi: 10.1364/OE.20.000960.

DOI:10.1364/OE.20.000960

PMID:22274443

Abstract

Three-dimensional fluorescence lifetime microscopy is achieved by combining wide-field fluorescence lifetime imaging with a remote optical refocusing method. As required for some applications in dynamic research for physics, chemistry, or biology, it is thereby not necessary to move the sample, i.e., the specimen is not disturbed during measurement. Using a fluorescent microsphere the performance of the system has been tested successfully with respect to three-dimensional fluorescence lifetime microscopy as well as time-resolved fluorescence spectroscopy.

摘要

三维荧光寿命显微镜是通过将宽场荧光寿命成像与远程光学重新聚焦方法相结合来实现的。根据物理、化学或生物学动态研究中某些应用的要求，因此无需移动样品，即在测量过程中不会干扰样本。使用荧光微球，该系统在三维荧光寿命显微镜以及时间分辨荧光光谱方面的性能已得到成功测试。