Ramos-Sánchez M C, Rodríguez-Torres A, Leal J A, Martín-Gil F J, Martín-Gil J
Departamento de Microbiología, Facultad de Medicina, Valladolid, Spain.
Biotechnol Prog. 1991 Nov-Dec;7(6):526-33. doi: 10.1021/bp00012a007.
The present work constitutes an entirely novel contribution in the scope of microbiology and especially in taxonomy, introducing thermolysis curves as a rapid method of characterization of fungal polysaccharides and bacterial lipopolysaccharides. The thermal analysis techniques applied were thermogravimetry and derivative thermogravimetry (TG-DTG), differential thermal analysis (DTA), and differential scanning calorimetry (DSC). Each thermogram of a sample is represented by one or a few temperatures and, in DSC, by complementary enthalpy data. The temperatures of the thermograms from structurally unknown polysaccharides are compared with those used as references, and thus, information on their composition, linkage types, and anomeric configuration can be deduced. The situation is more complicated for bacterial lipopolysaccharides, but in whatever mode, a structural estimation is always possible. In the course of the development and validation of the thermal method, structural findings on relative stabilities of linkage types (valuable in carbohydrate research) have been recognized and are therefore also described in this work.
本研究在微生物学领域，特别是在分类学方面做出了全新贡献，引入热解曲线作为表征真菌多糖和细菌脂多糖的快速方法。所应用的热分析技术包括热重法和微商热重法（TG-DTG）、差示热分析（DTA）和差示扫描量热法（DSC）。样品的每个热谱图由一个或几个温度表示，在DSC中还由互补的焓数据表示。将结构未知的多糖的热谱图温度与用作参考的温度进行比较，从而可以推断出其组成、连接类型和异头构型信息。对于细菌脂多糖，情况更为复杂，但无论采用何种方式，结构估计总是可行的。在热分析方法的开发和验证过程中，已经认识到连接类型相对稳定性的结构发现（在碳水化合物研究中有价值），因此本研究也对其进行了描述。
