Yu P, San K Y
Department of Chemical Engineering, Rice University, Houston, Texas 77251-1892.
Biotechnol Prog. 1992 Jan-Feb;8(1):25-9. doi: 10.1021/bp00013a005.
The effect of induction level of the bacteriocin release protein (BRP) on cell growth characteristics, protein expression, and protein release in a recombinant strain of Escherichia coli RR1 was investigated. Mitomycin C, the inducing agent, when added to the growth medium in moderate amounts (up to 200 ng/mL) was observed to enhance the release of periplasmic proteins from the cell to the fermentation broth substantially. The percentages of release of the proteins alpha-amylase and beta-lactamase were increased by factors of about 7 and 3, respectively, upon induction of the BRP. The percentage of alpha-amylase released into the broth increased from only about 5% to almost 50% with the aid of BRP. The cell growth curve and low extracellular activity of the cytoplasmic protein beta-galactosidase were indicative that cell lysis did not occur in an appreciable amount at a low induction level, with a mitomycin C concentration of less than 300 ng/mL.
研究了细菌素释放蛋白（BRP）的诱导水平对重组大肠杆菌RR1菌株的细胞生长特性、蛋白质表达和蛋白质释放的影响。观察到，诱导剂丝裂霉素C以适量（高达200 ng/mL）添加到生长培养基中时，可显著增强周质蛋白从细胞释放到发酵液中的能力。诱导BRP后，α-淀粉酶和β-内酰胺酶的蛋白质释放百分比分别提高了约7倍和3倍。借助BRP，释放到肉汤中的α-淀粉酶百分比从仅约5%增加到近50%。细胞生长曲线和细胞质蛋白β-半乳糖苷酶的低细胞外活性表明，在丝裂霉素C浓度低于300 ng/mL的低诱导水平下，细胞没有明显的裂解现象。
