Schiltz John F, Rustighi Alessandra, Tessari Michela A, Liu Jun, Braghetta Paola, Sgarra Riccardo, Stebel Marco, Bressan Giorgio M, Altruda Fiorella, Giancotti Vincenzo, Chada Kiran, Manfioletti Guidalberto
Department of Biochemistry, Robert Wood Johnson Medical School, University of Medicine and Dentistry of New Jersey, 675 Hoes Lane, Piscataway, NJ 08854, USA.
Biochem Biophys Res Commun. 2003 Oct 3;309(4):718-23. doi: 10.1016/j.bbrc.2003.08.062.
HMGA2(2) belongs to the high mobility group A (HMGA) family of architectural transcription factors which participate in a wide variety of nuclear processes ranging from transcription to recombination, playing an important role in chromatin remodelling. HMGA2 is expressed during embryogenesis but not by adult somatic tissues, yet it becomes re-expressed following neoplastic transformation. A role in development is underscored by the finding that the inactivation of the Hmga2 gene is responsible for the murine pygmy phenotype. To elucidate mechanisms that control HMGA2 expression, we have previously cloned the gene and identified functional elements involved in its regulation. In this paper, transgenic mice were generated to define genomic regions involved in Hmga2 developmental and tissue-specific transcriptional regulation. A genomic region from -8.1 to -3.7kb upstream from the initiation site has been found to recapitulate most of the spatial and temporal endogenous Hmga2 gene expression.
HMGA2(2)属于高迁移率族A（HMGA）家族的结构转录因子，该家族参与从转录到重组的多种核过程，在染色质重塑中发挥重要作用。HMGA2在胚胎发生过程中表达，但在成人体细胞组织中不表达，然而在肿瘤转化后它会重新表达。Hmga2基因的失活导致小鼠侏儒症表型，这一发现突出了其在发育中的作用。为了阐明控制HMGA2表达的机制，我们之前已克隆该基因并鉴定了参与其调控的功能元件。在本文中，我们构建了转基因小鼠以确定参与Hmga2发育和组织特异性转录调控的基因组区域。已发现起始位点上游-8.1至-3.7kb的基因组区域可重现内源性Hmga2基因大部分的时空表达。
