Davis Allison P, Symington Lorraine S
Department of Microbiology and Institute of Cancer Research, Columbia University College of Physicians and Surgeons, 701 W 168th St., New York, NY 10032, USA.
DNA Repair (Amst). 2003 Oct 7;2(10):1127-34. doi: 10.1016/s1568-7864(03)00121-6.
The RAD52 gene is essential for homology-dependent repair of double-strand breaks in Saccharomyces cerevisiae. Rad52 forms complexes with Rad51, replication protein A (RPA) or Rad59 and its presence is essential for the formation of Rad51-Rad52-Rad59 and RPA-Rad52-Rad59 complexes. The N-terminal region of Rad52, which is required for self-interaction to form a ring structure, is required for interaction with Rad59. Rad59 also shows self-interaction suggesting the formation of heteromeric and homomeric rings of Rad52 and Rad59. In wild-type cells, we propose the Rad51-Rad52-Rad59 complex is involved in conservative recombination events, including gene conversion and reciprocal recombination, whereas the Rad52-Rad59 complex participates in single-strand annealing.
RAD52基因对于酿酒酵母中双链断裂的同源依赖性修复至关重要。Rad52与Rad51、复制蛋白A（RPA）或Rad59形成复合物，并且其存在对于Rad51-Rad52-Rad59和RPA-Rad52-Rad59复合物的形成至关重要。Rad52的N端区域是形成环状结构的自我相互作用所必需的，也是与Rad59相互作用所必需的。Rad59也表现出自我相互作用，提示形成了Rad52和Rad59的异源和同源环。在野生型细胞中，我们认为Rad51-Rad52-Rad59复合物参与保守重组事件，包括基因转换和相互重组，而Rad52-Rad59复合物参与单链退火。
