半自动固相DNA测序
Semi-automated solid-phase DNA sequencing.
作者信息
Uhlén M, Hultman T, Wahlberg J, Lundeberg J, Bergh S, Pettersson B, Holmberg A, Ståhl S, Moks T
机构信息
Department of Biochemistry and Biotechnology, Royal Institute of Technology, Stockholm, Sweden.
出版信息
Trends Biotechnol. 1992 Jan-Feb;10(1-2):52-5. doi: 10.1016/0167-7799(92)90169-v.
Increasing the efficiency of DNA sequencing necessitates the development of systems which reduce the need for manual operations by integrating template preparation, sequencing reactions, product separation and detection. A semi-automated system, whereby PCR-amplified biotinylated genomic or plasmid DNA is immobilized on streptavidin-coated magnetic beads, has been developed.
提高DNA测序效率需要开发能够通过整合模板制备、测序反应、产物分离和检测来减少人工操作需求的系统。已经开发出一种半自动化系统，通过该系统，经PCR扩增的生物素化基因组或质粒DNA被固定在链霉亲和素包被的磁珠上。
Zotero 插件