Cook James R, Craig Fiona E, Swerdlow Steven H
Department of Pathology, University of Pittsburgh School of Medicine, Pittsburgh, Pennsylvania 15213, USA.
Mod Pathol. 2003 Sep;16(9):879-85. doi: 10.1097/01.MP.0000084630.64243.D1.
Recent reports have indicated that the neoplastic T cells of angioimmunoblastic T-cell lymphoma express CD10. It has been suggested that the demonstration of a CD10+ T-cell population may assist in establishing a diagnosis of angioimmunoblastic T-cell lymphoma and in distinguishing angioimmunoblastic T-cell lymphoma from other peripheral T-cell lymphomas. It has been unclear, however, whether this phenotypically unusual T-cell population might be present in other settings as well. In this report, we have retrospectively examined 64 cases of lymph node and solid tissue biopsies for the presence of CD10+ T cells using multicolor flow cytometry. Discrete populations of CD10+ T cells were found in 5 of 28 cases (18%) of reactive lymphoid hyperplasia, 4 of 17 cases (23%) of follicular lymphoma, and 9 of 19 cases (47%) of marginal zone B-cell lymphomas. The CD10+ T cells constituted 1-6% of total cells analyzed and </=14% of the total T-cell population. Using two-color immunohistochemical stains, many of the CD10+ PAX5-negative presumptive T cells were found to be located within germinal centers. These findings indicate that a normal small subset of CD10+ peripheral T cells exists and, at least when present in small numbers, should not be considered an indication of a T-cell neoplasm.
近期报告显示，血管免疫母细胞性T细胞淋巴瘤的肿瘤性T细胞表达CD10。有人提出，证实存在CD10+ T细胞群体可能有助于血管免疫母细胞性T细胞淋巴瘤的诊断，并有助于将其与其他外周T细胞淋巴瘤相鉴别。然而，尚不清楚这种表型异常的T细胞群体是否也可能存在于其他情况中。在本报告中，我们使用多色流式细胞术对64例淋巴结和实体组织活检样本进行回顾性检查，以确定是否存在CD10+ T细胞。在28例反应性淋巴组织增生中有5例（18%）、17例滤泡性淋巴瘤中有4例（23%）以及19例边缘区B细胞淋巴瘤中有9例（47%）发现了离散的CD10+ T细胞群体。CD10+ T细胞占所分析细胞总数的1%-6%，占总T细胞群体的≤14%。使用双色免疫组织化学染色发现，许多CD10+且PAX5阴性的推测性T细胞位于生发中心内。这些发现表明，正常情况下存在一小部分CD10+外周T细胞，并且至少当数量较少时，不应将其视为T细胞肿瘤的指征。
