Almasri N M, Iturraspe J A, Braylan R C
Department of Pathology, Immunology and Laboratory Medicine, University of Florida College of Medicine, Gainesville 32610-0275, USA.
Arch Pathol Lab Med. 1998 Jun;122(6):539-44.
CD10 is a proteolytic enzyme expressed on the surface of germinal center cells and lymphomas derived from these cells. There is a well-known association between CD10 expression and lymphomas of follicular center cell origin. However, the reported frequency of CD10 positivity in follicular lymphomas is widely variable, and no studies have addressed the importance of assessing the intensity of CD10 expression in the diagnosis of these tumors. In this study, we utilized flow cytometry to determine differences in CD10 expression in lymphomas and follicular hyperplasias.
Cell suspensions from 61 follicular lymphomas, 43 diffuse large B-cell lymphomas, and 44 lymph nodes with follicular hyperplasia were analyzed simultaneously with phycoerythrin-labeled anti-CD20 and fluorescein isothiocyanate-labeled anti-CD10.
CD10 expression was mainly observed on B cells and was detected in 89% of follicular lymphomas, 56% of diffuse large B-cell lymphomas, and 55% of lymph nodes showing follicular hyperplasia. In follicular hyperplasia, two subpopulations of B cells displaying dim and bright CD20 expression were recognized. CD10 expression was restricted to the bright CD20-positive cells, which accounted for an average of 16% of B cells. In CD10-positive follicular and diffuse large B-cell lymphoma cases, a significantly higher proportion of B cells (73%) coexpressed CD10. Furthermore, the intensity of CD10 expression in follicular lymphoma and diffuse large B-cell lymphoma was much higher than that of follicular hyperplasia.
Quantitative flow cytometry can detect significant differences in CD10 expression between normal follicular cells and follicular or diffuse large B-cell lymphoma. The use of CD10 intensity of expression as well as the fraction of CD10-expressing B cells should help distinguish reactive from neoplastic B-cell processes.
CD10是一种在生发中心细胞及其来源的淋巴瘤表面表达的蛋白水解酶。CD10表达与滤泡中心细胞来源的淋巴瘤之间存在众所周知的关联。然而，滤泡性淋巴瘤中CD10阳性的报道频率差异很大，且尚无研究探讨评估CD10表达强度在这些肿瘤诊断中的重要性。在本研究中，我们利用流式细胞术来确定淋巴瘤和滤泡增生中CD10表达的差异。
同时用藻红蛋白标记的抗CD20和异硫氰酸荧光素标记的抗CD10分析61例滤泡性淋巴瘤、43例弥漫性大B细胞淋巴瘤和44例有滤泡增生的淋巴结的细胞悬液。
CD10表达主要见于B细胞，在89%的滤泡性淋巴瘤、56%的弥漫性大B细胞淋巴瘤和55%有滤泡增生的淋巴结中检测到。在滤泡增生中，识别出显示CD20表达暗淡和明亮的两个B细胞亚群。CD10表达局限于明亮的CD20阳性细胞，这些细胞平均占B细胞的16%。在CD10阳性的滤泡性和弥漫性大B细胞淋巴瘤病例中，共表达CD10的B细胞比例显著更高（73%）。此外，滤泡性淋巴瘤和弥漫性大B细胞淋巴瘤中CD10表达强度远高于滤泡增生。
定量流式细胞术可检测正常滤泡细胞与滤泡性或弥漫性大B细胞淋巴瘤之间CD10表达的显著差异。使用CD10表达强度以及表达CD10的B细胞比例应有助于区分反应性与肿瘤性B细胞过程。
