Klymiuk Nikolai, Müller Mathias, Brem Gottfried, Aigner Bernhard
Institut für Tierzucht und Genetik, Veterinärmedizinische Universität Wien, A-1210 Vienna, Austria.
ApoGene Biotechnologie, D-86567 Hilgertshausen, Germany.
J Gen Virol. 2003 Oct;84(Pt 10):2729-2734. doi: 10.1099/vir.0.19284-0.
Prevention of cross-species infection of porcine endogenous retroviruses (PERV) is crucial for xenotransplantation. The potential risk of infection is caused by replication-competent PERV as well as by hybrid viruses derived from recombination events of distinct PERV genomes. Recently, human-tropic, replication-competent PERV genomes obtaining hybrid sequences have been observed. Here, complete polymorphism pattern analysis was performed on the full-length PERV gamma1 clones and on the complete envelope (env) gene sequences published to date. Several recombined full-length clones and a high number of different recombination patterns in the env gene were identified. In addition, recombinations with retroviral genomes not yet known were found. Thus, the potential risk of infection also exists for recombination products, including defective PERV loci.
预防猪内源性逆转录病毒（PERV）的跨物种感染对于异种移植至关重要。具有复制能力的PERV以及源自不同PERV基因组重组事件的杂交病毒会导致潜在的感染风险。最近，已观察到获得杂交序列的具有感染人类能力且具有复制能力的PERV基因组。在此，对全长PERV gamma1克隆以及迄今为止公布的完整包膜（env）基因序列进行了完整的多态性模式分析。鉴定出了几个重组的全长克隆以及env基因中大量不同的重组模式。此外，还发现了与尚未知晓的逆转录病毒基因组的重组。因此，包括有缺陷的PERV位点在内的重组产物也存在潜在的感染风险。
