C型嗜亲性猪内源性逆转录病毒感染性复制分子克隆的分离与鉴定
Isolation and characterization of an infectious replication-competent molecular clone of ecotropic porcine endogenous retrovirus class C.
作者信息
Preuss Thomas, Fischer Nicole, Boller Klaus, Tönjes Ralf R
机构信息
Paul-Ehrlich-Institut, Paul-Ehrlich-Strasse 51-59, D-63225 Langen, Germany.
出版信息
J Virol. 2006 Oct;80(20):10258-61. doi: 10.1128/JVI.01140-06.
Abstract
Xenotransplantation of pig organs is complicated by the existence of polytropic replication-competent porcine endogenous retroviruses (PERV) capable of infecting human cells. The potential for recombination between ecotropic PERV-C and human-tropic PERV-A and PERV-B adds another level of infectious risk. Proviral PERV-C were characterized in MAX-T cells derived from d/d haplotype miniature swine. Three proviruses were cloned from a genomic library. Clone PERV-C(1312) generated infectious particles after transfection into porcine ST-IOWA cells. Electron microscopy revealed the same morphologies of virions in MAX-T cells and in ST-IOWA cells infected with cell-free PERV-C(1312) particles, indicating that MAX-T cells harbor one functional PERV-C provirus.
摘要
猪器官的异种移植因存在能够感染人类细胞的多嗜性具有复制能力的猪内源性逆转录病毒（PERV）而变得复杂。亲嗜性PERV-C与人类嗜性PERV-A和PERV-B之间发生重组的可能性增加了另一层感染风险。在源自d/d单倍型小型猪的MAX-T细胞中对前病毒PERV-C进行了表征。从基因组文库中克隆了三种前病毒。克隆的PERV-C(1312)转染到猪ST-IOWA细胞后产生了感染性颗粒。电子显微镜显示MAX-T细胞和感染无细胞PERV-C(1312)颗粒的ST-IOWA细胞中病毒粒子的形态相同，表明MAX-T细胞含有一种功能性PERV-C前病毒。
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