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C型嗜亲性猪内源性逆转录病毒感染性复制分子克隆的分离与鉴定

Isolation and characterization of an infectious replication-competent molecular clone of ecotropic porcine endogenous retrovirus class C.

作者信息

Preuss Thomas, Fischer Nicole, Boller Klaus, Tönjes Ralf R

机构信息

Paul-Ehrlich-Institut, Paul-Ehrlich-Strasse 51-59, D-63225 Langen, Germany.

出版信息

J Virol. 2006 Oct;80(20):10258-61. doi: 10.1128/JVI.01140-06.

DOI:10.1128/JVI.01140-06
PMID:17005704
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC1617276/
Abstract

Xenotransplantation of pig organs is complicated by the existence of polytropic replication-competent porcine endogenous retroviruses (PERV) capable of infecting human cells. The potential for recombination between ecotropic PERV-C and human-tropic PERV-A and PERV-B adds another level of infectious risk. Proviral PERV-C were characterized in MAX-T cells derived from d/d haplotype miniature swine. Three proviruses were cloned from a genomic library. Clone PERV-C(1312) generated infectious particles after transfection into porcine ST-IOWA cells. Electron microscopy revealed the same morphologies of virions in MAX-T cells and in ST-IOWA cells infected with cell-free PERV-C(1312) particles, indicating that MAX-T cells harbor one functional PERV-C provirus.

摘要

猪器官的异种移植因存在能够感染人类细胞的多嗜性具有复制能力的猪内源性逆转录病毒（PERV）而变得复杂。亲嗜性PERV-C与人类嗜性PERV-A和PERV-B之间发生重组的可能性增加了另一层感染风险。在源自d/d单倍型小型猪的MAX-T细胞中对前病毒PERV-C进行了表征。从基因组文库中克隆了三种前病毒。克隆的PERV-C(1312)转染到猪ST-IOWA细胞后产生了感染性颗粒。电子显微镜显示MAX-T细胞和感染无细胞PERV-C(1312)颗粒的ST-IOWA细胞中病毒粒子的形态相同，表明MAX-T细胞含有一种功能性PERV-C前病毒。

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本文引用的文献

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The infectivity and host range of the ecotropic porcine endogenous retrovirus, PERV-C, is modulated by residues in the C-terminal region of its surface envelope protein.嗜亲性猪内源性逆转录病毒PERV-C的感染性和宿主范围受其表面包膜蛋白C末端区域残基的调控。
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Evidence and consequence of porcine endogenous retrovirus recombination.猪内源性逆转录病毒重组的证据及后果。
J Virol. 2004 Dec;78(24):13880-90. doi: 10.1128/JVI.78.24.13880-13890.2004.
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Determinants of high titer in recombinant porcine endogenous retroviruses.重组猪内源性逆转录病毒高滴度的决定因素
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Absence of replication-competent human-tropic porcine endogenous retroviruses in the germ line DNA of inbred miniature Swine.近交系小型猪生殖系DNA中无具有复制能力的嗜人猪内源性逆转录病毒。
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Identification of exogenous forms of human-tropic porcine endogenous retrovirus in miniature Swine.小型猪中人嗜性猪内源性逆转录病毒外源形式的鉴定
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