Oh S K, Nienow A W, al-Rubeai M, Emery A N
SERC Centre for Biochemical Engineering, School of Chemical Engineering, University of Birmingham, Edgbaston, U.K.
J Biotechnol. 1992 Feb;22(3):245-70. doi: 10.1016/0168-1656(92)90144-x.
TB/C3 mouse hybridoma cells have been grown at 2 controlled dO2 conditions by headspace and sparged oxygenation. Also a variety of sparging rates and sparger sizes and positions have been employed. Headspace oxygenation at dO2 levels from 5% to 100% of saturation give essentially the same performance as controls. Sparging is generally damaging to cells, the extent of damage decreasing with reduced sparging rate until at below about 0.02 vvm results equivalent to the unsparged conditions are obtained. Damage is clearly linked with bubble-cell interactions at the air-medium interface where bubbles bursting in clusters and of a size less than 5 mm appear to be the most lethal. When the interaction of air sparging with the agitator flow leads to an increase in the number of smaller bubbles and cluster bursts, cell damage is further increased. Pluronic F-68 reduces damage very significantly. Biological aspects are briefly discussed in the light of various biological tests. The practical implications of this work for large scale, free suspension cell culture are outlined.
TB/C3小鼠杂交瘤细胞已通过顶空和鼓泡充氧在两种受控的溶解氧（dO2）条件下培养。还采用了各种鼓泡速率、鼓泡器尺寸和位置。在饱和度的5%至100%的dO2水平下进行顶空充氧，其性能与对照基本相同。鼓泡通常对细胞有损害，损害程度随着鼓泡速率的降低而减小，直到低于约0.02 vvm（体积气体每分钟每体积培养液）时，可获得与未鼓泡条件相当的结果。损害显然与气液界面处的气泡-细胞相互作用有关，成簇破裂且尺寸小于5毫米的气泡似乎最具杀伤力。当鼓入的空气与搅拌器流动相互作用导致较小气泡数量增加和成簇破裂时，细胞损害会进一步加剧。普朗尼克F-68能显著降低损害。根据各种生物学测试简要讨论了生物学方面的问题。概述了这项工作对大规模自由悬浮细胞培养的实际意义。
