Narsa Reddy M, Tang Lin-Ya, Lee Tai-Lin, James Shen C-K
Institute of Molecular Biology, Academia Sinica, Nankang, Taipei 115, Taiwan, ROC.
Oncogene. 2003 Sep 18;22(40):6301-3. doi: 10.1038/sj.onc.1206650.
Like vertebrates, the genome of Drosophila melanogaster also contains methylated cytosines. However, the enzyme(s) responsible for this methylation has been elusive. By DNA transfection and sodium bisulfite sequencing, we show here that overexpression of dDnmt2, which is the only expressed and cloned Drosophila protein consisting of motifs conserved among the DNA cytosine methyltransferases, results in genomic DNA methylation of Drosophila S2 cells. The data provide the first evidence for dDnmt2 being one candidate gene encoding the Drosophila DNA methyltransferase(s).
与脊椎动物一样，黑腹果蝇的基因组中也含有甲基化的胞嘧啶。然而，负责这种甲基化的酶一直难以捉摸。通过DNA转染和亚硫酸氢钠测序，我们在此表明，dDnmt2的过表达导致果蝇S2细胞的基因组DNA甲基化，dDnmt2是唯一表达并克隆的果蝇蛋白，其包含在DNA胞嘧啶甲基转移酶中保守的基序。这些数据为dDnmt2作为编码果蝇DNA甲基转移酶的一个候选基因提供了首个证据。
