Salzberg Adi, Fisher Ohad, Siman-Tov Rama, Ankri Serge
Department of Genetics and the Rappaport Family Institute for Research in the Medical Sciences, Technion, Israel.
Biochem Biophys Res Commun. 2004 Sep 17;322(2):465-9. doi: 10.1016/j.bbrc.2004.07.134.
The genome of Drosophila melanogaster contains methylated cytosines. Recent studies indicate that DNA methylation in the fruit fly depends on one DNA methyltransferase, dDNMT2. No obvious phenotype is associated with the downregulation of this DNA methyltransferase. Thus, identifying the target sequences methylated by dDNMT2 may constitute the first step towards understanding the biological functions of this enzyme. We used anti-5-methylcytosine antibodies as affinity column to identify the methylated sequences in the genome of adult flies. Our analysis demonstrates that components of retrotransposons and repetitive DNA sequences are putative substrates for dDNMT2. The methylation status of DNA encoding Gag, a protein involved in delivering the transposition template to its DNA target, was confirmed by sodium bisulfite sequencing.
黑腹果蝇的基因组含有甲基化的胞嘧啶。最近的研究表明，果蝇中的DNA甲基化依赖于一种DNA甲基转移酶，即dDNMT2。该DNA甲基转移酶的下调未表现出明显的表型。因此，鉴定由dDNMT2甲基化的靶序列可能是理解该酶生物学功能的第一步。我们使用抗5-甲基胞嘧啶抗体作为亲和柱来鉴定成年果蝇基因组中的甲基化序列。我们的分析表明，逆转座子和重复DNA序列的成分是dDNMT2的假定底物。通过亚硫酸氢盐测序证实了编码Gag（一种参与将转座模板传递至其DNA靶标的蛋白质）的DNA的甲基化状态。
