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固定在κ-卡拉胶凝胶中的保加利亚乳杆菌的稳定性

Stability of Lactobacillus bulgaricus immobilized in kappa-carrageenan gels.

作者信息

Büyükgüngör H

机构信息

Environmental Engineering Department, Ondokuz Mayìs University, Kurupelit/Samsun, Turkey.

出版信息

J Chem Technol Biotechnol. 1992;53(2):173-5. doi: 10.1002/jctb.280530211.

DOI:10.1002/jctb.280530211
PMID:1368012
Abstract

The future application of immobilized microorganism techniques will depend on the development of systems which are technologically applicable on an industrial scale. These techniques must permit high microbial concentrations and must allow mass transfer to take place with low diffusional limitations. In addition, the mechanical separation of the immobilized microorganisms must be achieved economically. Kappa-carrageenan was used as an ionotropic gel carrier for the immobilization of Lactobacillus bulgaricus (ATCC 11842), and the effects of gel stability on productivity and the rate of product formation were investigated. kappa-carrageenan gels had higher mechanical and chemical stability than alginate gels. The storage stability of microorganisms immobilized in kappa-carrageenan was good enough to retain biocatalytic activity during prolonged storage at 4 degrees C.

摘要

固定化微生物技术的未来应用将取决于在工业规模上具有技术适用性的系统的开发。这些技术必须允许高微生物浓度,并且必须在低扩散限制下进行传质。此外,必须经济地实现固定化微生物的机械分离。κ-卡拉胶用作固定保加利亚乳杆菌(ATCC 11842)的离子型凝胶载体,并研究了凝胶稳定性对生产率和产物形成速率的影响。κ-卡拉胶凝胶比藻酸盐凝胶具有更高的机械和化学稳定性。固定在κ-卡拉胶中的微生物的储存稳定性足以在4℃长期储存期间保持生物催化活性。

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