Gardin H, Pauss A
Département de Génie Chimique, Université de Technologie de Compiègne, BP 20-529, France.
Appl Microbiol Biotechnol. 2001 Aug;56(3-4):517-23. doi: 10.1007/s002530000581.
Alginate and kappa-carrageenan gels were tested as bead materials for the co-immobilization of anaerobic and aerobic microorganisms for the mineralization of 2,4,6-trichlorophenol under air-limited conditions. Chemical, mechanical and thermal culture constraints were pre-defined and the gel resistances were established. Alginate was quickly eliminated because of its chemical instability in the culture media. In anaerobic conditions, the microorganisms transformed the substrates into CH4 and CO2. The kappa-carrageenan gel did not enable these gases to diffuse. They remained as bubbles in the core of the beads and made the beads float. Gelatin was added to kappa-carrageenan in order to change the carrier properties. No biogas bubbles appeared in the bead core during the cultures in anaerobic conditions and the beads reacted well to the culture conditions in the reactor. The co-immobilization of the anaerobic and aerobic microbial communities was successfully performed with the kappa-carrageenan/gelatin gel (2% (w/w) of each polymer). The biological activities, measured by the impedancemetry technique, were preserved in the beads.
测试了藻酸盐和κ-卡拉胶凝胶作为珠粒材料，用于在空气受限条件下共固定厌氧和好氧微生物，以实现2,4,6-三氯苯酚的矿化。预先定义了化学、机械和热培养限制条件，并确定了凝胶阻力。藻酸盐由于其在培养基中的化学不稳定性而很快被淘汰。在厌氧条件下，微生物将底物转化为CH4和CO2。κ-卡拉胶凝胶无法使这些气体扩散。它们以气泡形式留在珠粒核心，使珠粒漂浮。向κ-卡拉胶中添加明胶以改变载体特性。在厌氧条件下培养期间，珠粒核心未出现沼气气泡，且珠粒对反应器中的培养条件反应良好。使用κ-卡拉胶/明胶凝胶（每种聚合物2%（w/w））成功实现了厌氧和好氧微生物群落的共固定。通过阻抗测量技术测得的生物活性在珠粒中得以保留。
