Moldes A B, Alonso J L, Parajó J C
Department of Chemical Engineering, Faculty of Science, University of Vigo, Campus Ourense, As Lagoas, 32004 Ourense, Spain.
Bioprocess Biosyst Eng. 2003 Jul;25(6):357-63. doi: 10.1007/s00449-002-0316-7. Epub 2003 Feb 19.
The physicochemical properties (capacity, kinetics and selectivity) of the ion exchange resins Amberlite IRA900, IRA400, IRA96 and IRA67 were determined to evaluate their comparative suitability for lactic acid recovery. Both the kinetics of lactic acid sorption from aqueous solutions and the equilibrium were assessed using mathematical models, which provided a close interpretation of the experimental results. The best resins (Amberlite IRA96 and IRA67) were employed in further fixed-bed operation using aqueous lactic acid solutions as feed. In this set of experiments, parameters such as capacity, regenerant consumption, percentage of lactic acid recovery and product concentration were measured. Amberlite IRA67, a weak base resin, was selected for lactic acid recovery from SSF (simultaneous saccharification and fermentation) broths. Owing to the presence of nutrients and ions other than lactate, a slightly decreased capacity was determined when using SSF media instead aqueous lactic acid solutions, but quantitative lactic acid recoveries at constant capacities were obtained in four sequential load/regeneration cycles.
测定了离子交换树脂Amberlite IRA900、IRA400、IRA96和IRA67的物理化学性质（容量、动力学和选择性），以评估它们对乳酸回收的相对适用性。使用数学模型评估了从水溶液中吸附乳酸的动力学和平衡，该模型对实验结果给出了相近的解释。使用乳酸水溶液作为进料，采用最佳树脂（Amberlite IRA96和IRA67）进行进一步的固定床操作。在这组实验中，测量了容量、再生剂消耗、乳酸回收率和产物浓度等参数。选择弱碱性树脂Amberlite IRA67从同步糖化发酵（SSF）发酵液中回收乳酸。由于除乳酸盐外还存在营养物质和离子，因此使用SSF培养基代替乳酸水溶液时，测定的容量略有下降，但在四个连续的负载/再生循环中，在恒定容量下实现了乳酸的定量回收。
