Eiteman M A, Gainer J L
Center for Bioprocess and Product Development, Department of Chemical Engineering, University of Virginia, Charlottesville 22903-2442.
Bioseparation. 1991;2(1):31-41.
A mathematical model has been developed to predict partition coefficients for aqueous two-phase systems. The model is based on a previously-developed equation for partitioning which arises from an osmotic pressure viral expansion. The model suggests that the properties of importance are the concentration difference of one of the phase-forming components, such as a polymer, and the hydrophobicity of the solute relative to the hydrophobic-hydrophilic difference between the two phases. Several two-phase systems have been studied, with a particular emphasis on the poly(ethylene glycol)/magnesium sulfate system. Numerous solutes, including peptides, were used in this system and their partition coefficients show good agreement with the model.
已经开发了一个数学模型来预测双水相系统的分配系数。该模型基于先前开发的一个分配方程，该方程源于渗透压病毒膨胀。该模型表明，重要的性质是一种成相组分（如聚合物）的浓度差，以及溶质相对于两相之间疏水-亲水平衡的疏水性。已经研究了几种双水相系统，特别强调了聚乙二醇/硫酸镁系统。在该系统中使用了包括肽在内的多种溶质，它们的分配系数与模型显示出良好的一致性。
