Lloyd L L, Warner F P, Kennedy J F
Polymer Laboratories Ltd., Church Stretton, Shropshire, UK.
Bioseparation. 1991;2(4):207-15.
The ability to resolve and purify synthetic oligonucleotides by high performance anion exchange chromatography was evaluated using two wide pore polymeric HPLC matrices. The materials used are rigid macroporous copolymers which have a fully quaternised polyethyleneimine coating to provide a strong anion exchange, quaternary amine, functionality. Oligomers of poly(rA), poly(rC) and RNA produced by alkaline hydrolysis of the polymers were chromatographed to evaluate the selectivity of the system prior to the analysis of synthetic oligonucleotides produced using a commercial oligonucleotide synthesizer.
使用两种大孔聚合物高效液相色谱基质评估了通过高效阴离子交换色谱法分离和纯化合成寡核苷酸的能力。所使用的材料是刚性大孔共聚物，其具有完全季铵化的聚乙烯亚胺涂层，以提供强阴离子交换季胺官能团。在分析使用商业寡核苷酸合成仪生产的合成寡核苷酸之前，对通过聚合物的碱性水解产生的聚（rA）、聚（rC）和RNA的寡聚物进行色谱分析，以评估该系统的选择性。
