De Bellis G, Invernizzi L, Debernardi S, Pergolizzi R, Luzzana M
Istituto di Tecnologie, Biomediche Avanzate, Segrate, Italy.
Biotechniques. 1995 Aug;19(2):230-4.
Biotinylated oligonucleotides combined with streptavidin-coated magnetic beads are commonly used in current molecular biology. Their quality and the level of incorporated biotin are essential for yielding good results in either solid-phase DNA sequencing or solid-phase purification procedures. This paper presents a very simple analytical test using anion-exchange HPLC and avidin to ascertain the quality of biotinylated oligonucleotides and to predetermine their ability to bind to avidin, which is a prerequisite for functionality in some solid-phase methods.
生物素化寡核苷酸与链霉亲和素包被的磁珠相结合，在当前分子生物学中被广泛使用。它们的质量和生物素掺入水平对于在固相DNA测序或固相纯化程序中获得良好结果至关重要。本文介绍了一种非常简单的分析测试方法，使用阴离子交换高效液相色谱法和抗生物素蛋白来确定生物素化寡核苷酸的质量，并预先确定它们与抗生物素蛋白结合的能力，这是某些固相方法发挥功能的前提条件。
