Nixon L, Koval C A, Xu L, Noble R D, Slaff G S
Department of Chemistry and Biochemistry, University of Colorado, Boulder 80309-0215.
Bioseparation. 1991;2(4):217-30.
Liquid fluidized beds containing porous magnetic ion-exchange particles with densities ca. 1.03-1.16 g mL-1 were examined. The effect of magnetic stabilization was studied, both in terms of bed physical characteristics and sorptive behavior. Maximum applied magnetic field strength was approximately 200 oersted. Breakthrough and pulse analyses were carried out with protein and acetone solutions, respectively, with liquid flow rates ranging from approximately 1 to 3 cm min-1. Acetone pulses in columns containing 7 mL of particles had plate numbers ranging from 2.5 to 18 for magnetically stabilized beds and from 7.8 to 20 for non-stabilized fluidized beds. Under any particular set of conditions, magnetic stabilization always resulted in poorer efficiency, both in pulse analyses and in protein breakthrough experiments.
对含有密度约为1.03 - 1.16 g mL⁻¹的多孔磁性离子交换颗粒的液体流化床进行了研究。从床层物理特性和吸附行为两方面研究了磁稳定化的效果。最大施加磁场强度约为200奥斯特。分别用蛋白质溶液和丙酮溶液进行了穿透和脉冲分析，液体流速范围约为1至3 cm min⁻¹。在装有7 mL颗粒的柱中，对于磁稳定化床，丙酮脉冲的塔板数范围为2.5至18，对于未稳定化的流化床，塔板数范围为7.8至20。在任何特定的条件组下，无论是在脉冲分析还是蛋白质穿透实验中，磁稳定化总是导致效率较低。
