Draeger N M, Chase H A
Department of Chemical Engineering, University of Cambridge, UK.
Bioseparation. 1991;2(2):67-80.
The adsorption, in a liquid fluidized bed, of Bovine Serum Albumin (BSA), onto an ion-exchange absorbent, Q-Sepharose Fast Flow, in the presence of Alcaligenes eutrophus cells, has been studied. The expansion of the fluidized bed is greater in the presence than in the absence of cells and obeys the laws of Richardson and Zaki. The effect of cell concentration on the equilibrium adsorption characteristics of the adsorbent has been assessed. The rate of adsorption of BSA onto the adsorbent has been studied in a batch stirred tank, and a fluidized bed system both in the presence and absence of cells. Comparisons have been made with the adsorption of human immunoglobulin G (human IgG), onto an affinity adsorbent, Protein A Sepharose CL-4B. The data from the fluidized bed breakthrough tests have been used to assess the validity of a theoretical model adapted from one that predicts the performance of the adsorption phase in the absence of cells in fixed bed systems. Tests have been done on the washing phase in the fluidized bed adsorption system to establish the most efficient method of washing cells and unadsorbed protein out of the bed.
研究了在液体流化床中，在嗜碱产碱杆菌细胞存在的情况下，牛血清白蛋白（BSA）在离子交换吸附剂Q-Sepharose Fast Flow上的吸附情况。存在细胞时流化床的膨胀比不存在细胞时更大，且遵循理查森和扎基定律。评估了细胞浓度对吸附剂平衡吸附特性的影响。在间歇搅拌釜以及存在和不存在细胞的流化床系统中，研究了BSA在吸附剂上的吸附速率。将其与人类免疫球蛋白G（人IgG）在亲和吸附剂Protein A Sepharose CL-4B上的吸附进行了比较。流化床穿透试验的数据已用于评估一个理论模型的有效性，该模型改编自一个预测固定床系统中不存在细胞时吸附阶段性能的模型。对流化床吸附系统中的洗涤阶段进行了测试，以确定将细胞和未吸附的蛋白质从床中洗出的最有效方法。
