Shirasugi N, Misaki A
Department of Food and Nutrition, Faculty of Science of Living, Osaka City University, Japan.
Biosci Biotechnol Biochem. 1992 Jan;56(1):29-33. doi: 10.1271/bbb.56.29.
A cell-wall preparation from the cells of Elsinoe leucospila, which produces elsinan extracellularly when grown on sucrose or glucose-potato extract medium, was fractionated systematically. The heteropolysaccharide that was released by treatment with Actinase E digestion, comprised D-mannose, D-galactose, and D-glucose (molar ratio, 1.5:1.0:0.1). Methylation, mild acid hydrolysis, and 13C-NMR studies suggested that the polysaccharide contains a backbone of alpha-(1----6)-linked D-mannose residues having two kinds of side chains, one attached at the O-4 with single or short beta-(1----6)-linked D-galactofuranosyl residues, and the other attached at O-2 with short side chains, most probably, of alpha-(1----3)-linked D-mannopyranosyl residues. A moderately branched D-glucan fraction, obtained from the cold alkali extract, was fractionated to give an antitumor-active purified beta-(1----3)-glucan having branches of single beta-D-glucosyl groups, one out of eight D-glucose residues being substituted at the O-6.
从白星痂圆孢菌（Elsinoe leucospila）细胞制备的细胞壁制剂，该菌在蔗糖或葡萄糖 - 马铃薯提取物培养基上生长时可在细胞外产生痂圆孢菌素，对其进行了系统分级分离。用放线酶E消化处理后释放的杂多糖包含D - 甘露糖、D - 半乳糖和D - 葡萄糖（摩尔比为1.5:1.0:0.1）。甲基化、温和酸水解和13C - NMR研究表明，该多糖含有α-(1→6)连接的D - 甘露糖残基主链，具有两种侧链，一种在O - 4处连接单个或短的β-(1→6)连接的D - 呋喃半乳糖基残基，另一种在O - 2处连接短侧链，很可能是α-(1→3)连接的D - 吡喃甘露糖基残基。从冷碱提取物中获得的中度分支的D - 葡聚糖级分被分级分离，得到一种具有抗肿瘤活性的纯化β-(1→3) - 葡聚糖，其具有单个β - D - 葡萄糖基分支，八个D - 葡萄糖残基中有一个在O - 6处被取代。
