Aono R, Ogino H, Horikoshi K
Department of Bioengineering, Faculty of bioscience and Biotechnology, Tokyo Institute of Technology, Yokohama, Japan.
Biosci Biotechnol Biochem. 1992 Jan;56(1):48-53. doi: 10.1271/bbb.56.48.
A facultative alkaliphilic strain of Bacillus sp. C-125 grown at alkaline pH had many sinuous peritrichous flagella and was highly motile. However, most of the cells grown initially at pH 7 were non-motile and possessed few straight flagella. The amount of flagellin was low when the organism was grown at pH 7, suggesting that non-motility is due to poor synthesis of flagellin. The molecular mass of the flagellin was 37 kDa and the isoelectric point was pH 5.0. The amino acid composition of the flagellin was similar to that found in the flagellin from neutrophilic Bacillus subtilis 168.
在碱性pH条件下生长的芽孢杆菌属嗜碱兼性菌株C-125有许多蜿蜒的周生鞭毛，且运动性很强。然而，最初在pH 7条件下生长的大多数细胞不具运动性，且只有很少的直鞭毛。当该生物体在pH 7条件下生长时，鞭毛蛋白的量很低，这表明不运动是由于鞭毛蛋白合成不佳所致。鞭毛蛋白的分子量为37 kDa，等电点为pH 5.0。鞭毛蛋白的氨基酸组成与嗜中性枯草芽孢杆菌168的鞭毛蛋白相似。
