Martinez R J, Gordee E Z
J Bacteriol. 1966 Feb;91(2):870-5. doi: 10.1128/jb.91.2.870-875.1966.
Martinez, R. J. (University of California, Los Angeles), and E. Z. Gordee. Formation of bacterial flagella. I. Demonstration of a functional flagellin pool in Spirillum serpens and Bacillus subtilis. J. Bacteriol. 91:870-875. 1966-Exponentially growing cultures of Spirillum serpens and Bacillus subtilis regained motility and flagella within one generation after mechanical deflagellation. Regeneration of flagella occurred in both cultures in the presence of chloramphenicol at concentrations shown to inhibit flagellin synthesis. Cells labeled with C(14)-amino acids regenerated radioactive flagella in the presence of chloramphenicol. A conditional mutant of S. serpens (T-45) was isolated. This strain did not produce flagella when grown at 45 C, but formed the organelles upon temperature shift to 30 C, even in the presence of chloramphenicol. A reduction of intracellular antibody-precipitable flagellin counts in labeled S. serpens T-45 occurred concomitant with the generation of flagella at 30 C. The data suggest that the flagella of S. serpens and B. subtilis are formed from a pool of intracellular flagellin proteins.
马丁内斯，R. J.（加利福尼亚大学洛杉矶分校）和E. Z. 戈尔迪。细菌鞭毛的形成。I. 证明在蜿蜒螺菌和枯草芽孢杆菌中存在功能性鞭毛蛋白库。《细菌学杂志》91:870 - 875。1966年——在机械去除鞭毛后，蜿蜒螺菌和枯草芽孢杆菌的指数生长期培养物在一代时间内恢复了运动性并重新长出鞭毛。在氯霉素存在的情况下，两种培养物中的鞭毛都能再生，而氯霉素的浓度已被证明可抑制鞭毛蛋白的合成。用C(14) - 氨基酸标记的细胞在氯霉素存在的情况下能再生出放射性鞭毛。分离出了蜿蜒螺菌的一个条件突变体（T - 45）。该菌株在45℃生长时不产生鞭毛，但即使在氯霉素存在的情况下，温度转移到30℃时也会形成这些细胞器。在30℃时，标记的蜿蜒螺菌T - 45中细胞内可被抗体沉淀的鞭毛蛋白数量减少，同时伴随着鞭毛的产生。数据表明，蜿蜒螺菌和枯草芽孢杆菌的鞭毛是由细胞内鞭毛蛋白库形成的。
