用于蛋白质纯化的差异盐促进色谱法。
Differential salt-promoted chromatography for protein purification.
作者信息
Scopes R K, Porath J
机构信息
Centre for Protein and Enzyme Technology, La Trobe University, Bundoora, Victoria, Australia.
出版信息
Bioseparation. 1990;1(1):3-7.
A range of hydrophobic-type adsorbents for protein chromatography has been screened for the binding, at high salt concentrations, of 10 enzymes from a bacterial extract. Adsorbents were chosen for tandem chromatography, in which the first adsorbent removed much of the protein, and the second and subsequent columns bound the desired enzymes. Simple schemes for isolating Zymomonas mobilis and yeast alcohol dehydrogenases are described, in which the enzymes are affinity eluted by NAD+.
已筛选出一系列用于蛋白质色谱分析的疏水型吸附剂，以研究其在高盐浓度下对细菌提取物中10种酶的结合能力。选择吸附剂用于串联色谱分析，其中第一种吸附剂去除大部分蛋白质，而第二种及后续柱则结合所需的酶。文中描述了分离运动发酵单胞菌和酵母乙醇脱氢酶的简单方案，其中酶通过NAD+进行亲和洗脱。
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