Möller J, Niehoff J, Hotop S, Dors M, Schügerl K
Institut für Technische Chemie, Universität Hannover, Federal Republic of Germany.
Appl Microbiol Biotechnol. 1992 May;37(2):157-63. doi: 10.1007/BF00178163.
The influence of stirrer speed in the third preculture on the performance of penicillin V production by Penicillium chrysogenum in complex medium in a 100-l air-lift tower loop reactor was investigated. The process performance in the main culture was improved by increasing the stirrer speed from 500 to 750 rpm: the pellet size was reduced to half, the cell growth was influenced only slightly, but the production phase was extended considerably, and the final penicillin concentration was increased from 5.1 g l-1 to 10.4 g l-1.
研究了在100升气升式塔式环流反应器中，搅拌速度对产黄青霉在复合培养基中生产青霉素V性能的影响。通过将搅拌速度从500转/分钟提高到750转/分钟，主培养阶段的工艺性能得到改善：颗粒尺寸减小到原来的一半，细胞生长仅受到轻微影响，但生产阶段显著延长，最终青霉素浓度从5.1克/升提高到10.4克/升。
