Kaneko T, Kudo T, Horikoshi K
Riken Institute, Saitama, Japan.
Agric Biol Chem. 1990 Jan;54(1):197-201.
Using 12 chimeric cyclomaltodextrin glucanotransferases (CGTases) constructed from two genes, the CGTase gene from the alkalophilic Bacillus sp. strain No. 38-2 (CGTase 38-2 gene) and the CGTase gene from the alkalophilic Bacillus sp. strain No. 17-1 (CGTase 17-1 gene), we compared the effect of those chimeric enzymes on cyclodextrin (CD) production, especially on the composition of the CDs produced. It was found that the N-terminal and the C-terminal segments were important for CD production. Chimeric enzymes that contained the N-terminal and the C-terminal segments derived from CGTase 38-2 produced large amounts of CD, and especially a higher proportion of alpha-CD than those of other chimeric enzymes.
利用由两个基因构建的12种嵌合环糊精葡聚糖转移酶（CGTases），即嗜碱芽孢杆菌38 - 2菌株的CGTase基因（CGTase 38 - 2基因）和嗜碱芽孢杆菌17 - 1菌株的CGTase基因（CGTase 17 - 1基因），我们比较了这些嵌合酶对环糊精（CD）生产的影响，特别是对所生产CD组成的影响。发现N端和C端片段对CD生产很重要。含有源自CGTase 38 - 2的N端和C端片段的嵌合酶产生大量的CD，尤其是与其他嵌合酶相比，α - CD的比例更高。
