来自黄杆菌属U-188菌株的肌酸酶基因在大肠杆菌中的克隆与表达。
Cloning and expression of the creatinase gene from Flavobacterium sp. U-188 in Escherichia coli.
作者信息
Koyama Y, Kitao S, Yamamoto-Otake H, Suzuki M, Nakano E
机构信息
Research and Development Division, Kikkoman Corporation, Chiba, Japan.
出版信息
Agric Biol Chem. 1990 Jun;54(6):1453-7.
PMID:1368564
Abstract
The gene coding for creatinase (creatine amidinohydrolase, EC 3.5.3.3) was isolated from Flavobacterium sp. U-188. The primary structure of creatinase deduced from the nucleotide sequence showed a protein (molecular weight, 42, 651) composed of 378 amino acids. The creatinase gene was over-expressed in Escherichia coli under the control of the lac promoter and the amount of this enzyme was over 20% of the soluble protein in the cell.
摘要
编码肌酸酶（肌酸脒基水解酶，EC 3.5.3.3）的基因是从黄杆菌属菌株U - 188中分离得到的。根据核苷酸序列推导的肌酸酶一级结构显示该蛋白（分子量为42,651）由378个氨基酸组成。肌酸酶基因在乳糖启动子的控制下于大肠杆菌中过量表达，该酶的量超过细胞中可溶性蛋白的20%。
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