在重组大肠杆菌中生产新型抗叠氮化钠肌酸脒基水解酶。
Production of novel NaN3-resistant creatine amidinohydrolase in recombinant Escherichia coli.
作者信息
Liu Song, Dai Jun, Kang Zhen, Li Jianghua, Chen Jian, Du Guocheng
机构信息
a The Key Laboratory of Industrial Biotechnology; Ministry of Education; Jiangnan University ; Wuxi , China.
出版信息
Bioengineered. 2015;6(4):248-50. doi: 10.1080/21655979.2015.1052919.
Abstract
Creatinase (creatine amidinohydrolase), an important medical enzyme, has been used for clinical diagnosis of renal function because of its high substrate specificity. Recently, we successfully cloned a NaN3-resistant creatinase encoding gene from Arthrobacter nicotianae. By optimizing the cultivation process, we realized its high-level expression in Escherichia coli. In this addendum, production of this NaN3-resistant creatinase in E. coli and future research were further discussed.
摘要
肌酸酶（肌酸脒基水解酶）是一种重要的医学酶，因其高底物特异性而被用于肾功能的临床诊断。最近，我们成功地从烟草节杆菌中克隆了一个耐NaN₃的肌酸酶编码基因。通过优化培养过程，我们实现了它在大肠杆菌中的高水平表达。在本附录中，进一步讨论了这种耐NaN₃肌酸酶在大肠杆菌中的生产及未来的研究。
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