Kohmura M, Nio N, Ariyoshi Y
Central Research Laboratories, Ajinomoto Co., Inc., Kawasaki, Japan.
Agric Biol Chem. 1990 Sep;54(9):2219-24.
The sweet protein monellin consists of two noncovalently associated polypeptide chains, the A chain of 44 amino acid residues and the B chain of 50 residues. Two different primary structures have been reported for each of these chains. The complete amino acid sequence of monellin was determined by a combination of FAB- and ESI-mass spectrometry, and by automatic Edman degradation.
甜味蛋白莫内林由两条非共价结合的多肽链组成，A链有44个氨基酸残基，B链有50个残基。已报道这两条链各自有两种不同的一级结构。莫内林的完整氨基酸序列通过快原子轰击质谱法和电喷雾电离质谱法相结合，并通过自动埃德曼降解法确定。
