Hidaka T, Hara O, Imai S, Anzai H, Murakami T, Nagaoka K, Seto H
Institute of Applied Microbiology, University of Tokyo, Japan.
Agric Biol Chem. 1990 Aug;54(8):2121-5.
One of the three C-P bond formation steps, defined as step 5 in the bialaphos (BA) biosynthetic pathway, was analyzed using a new BA non-producing mutant NP71. The mutant was derived from a BA producer by gene replacement of an unidentified region next to the gene responsible for the step 5 deficiency of the mutant NP213, obtained by conventional mutation procedures. Biochemical analysis of these two mutants indicated that NP71 was defective in the formation of carboxyphosphonoenolpyruvate (CPEP), while NP213 lacked the enzyme CPEP phosphonomutase, which catalyzed the intramolecular rearrangement of CPEP.
双丙氨膦（BA）生物合成途径中的三个C-P键形成步骤之一（定义为步骤5），使用新的不产生BA的突变体NP71进行了分析。该突变体是通过常规诱变程序获得的、负责突变体NP213步骤5缺陷的基因旁边一个未鉴定区域的基因替换，从BA产生菌衍生而来。对这两个突变体的生化分析表明，NP71在羧基膦酰烯醇丙酮酸（CPEP）形成方面存在缺陷，而NP213缺乏催化CPEP分子内重排的CPEP膦酸变位酶。
