抗牛流行热病毒单克隆抗体的制备与鉴定

Establishment and characterization of monoclonal antibodies against bovine ephemeral fever virus.

作者信息

Eto N, Yamada K, Koga A, Shirahata S, Murakami H

机构信息

Department of Food Science and Technology, Faculty of Agriculture, Kyushu University, Fukuoka, Japan.

出版信息

Agric Biol Chem. 1991 Jan;55(1):167-72.

PMID:1368660

Abstract

We established fourteen monoclonal antibodies (MAbs) reactive to bovine ephemeral fever virus YHL strain, and characterized six representatives including three IgG1s (YG3/4, YG5/8, and YG6/7) and three IgMs (YM4/9, YM2/6, and YM6/8). Among them, YG3/4 and YM4/9 gave especially strong reactivities to the virus. YM4/9 reacted specifically with a 43K antigen of the virus, corresponding to the matrix protein 1. The other MAbs reacted most strongly with the 43K antigen, but also reacted with unknown 23K and 21K antigens. By a simultaneous two-site method using YM4/9 and YG3/4, it was possible to detect 10(4.10)TCID50/ml of the virus, in the presence of serum.

摘要

我们制备了14种对牛暂时热病毒YHL株有反应的单克隆抗体，并对6种代表性抗体进行了特性分析，其中包括3种IgG1（YG3/4、YG5/8和YG6/7）和3种IgM（YM4/9、YM2/6和YM6/8）。其中，YG3/4和YM4/9对该病毒的反应特别强烈。YM4/9与病毒的一种43K抗原特异性反应，该抗原对应于基质蛋白1。其他单克隆抗体与43K抗原反应最强，但也与未知的23K和21K抗原反应。通过使用YM4/9和YG3/4的同步双位点法，在有血清存在的情况下，可以检测到10(4.10)TCID50/ml的病毒。