Zakrzewski H, Cybinski D H, Walker P J
CSIRO Division of Tropical Animal Production, Indooroopilly, Queensland, Australia.
J Immunol Methods. 1992 Jul 6;151(1-2):289-97. doi: 10.1016/0022-1759(92)90129-h.
A blocking ELISA (B/ELISA) for detecting antibodies to bovine ephemeral fever virus (BEFV) in cattle is described. In this test, the binding capacity of a monoclonal antibody specific for an epitope on antigenic site G1 of the BEF virus glycoprotein is blocked in the presence of positive serum. The sensitivity of the B/ELISA was compared with the virus neutralisation (VN) test using a total of 380 sera from cattle. Of these, 118 were from an area known to be free of bovine ephemeral fever, 181 from naturally and experimentally BEFV-infected cattle, 33 sequential serum samples from a sentinel steer from which Berrimah virus (BERV) had been isolated, 9 from a sentinel cow from which Kimberley virus (KIMV) was isolated and a panel of 39 sera supplied as a blind trial. The B/ELISA results overall compared favourably with those of the VN tests. The monospecificity of the test was demonstrated using hyperimmune mouse ascitic fluid to other BEF serogroup viruses, namely KIM and BER viruses and the results showed no significant cross-reaction. The greater simplicity and sensitivity of the test when compared with the VN test makes it the preferred test for the diagnosis and monitoring of clinical bovine ephemeral fever.
本文描述了一种用于检测牛血清中牛流行热病毒（BEFV）抗体的阻断酶联免疫吸附测定法（B/ELISA）。在该试验中，在阳性血清存在的情况下，针对BEF病毒糖蛋白抗原位点G1上一个表位的单克隆抗体的结合能力被阻断。使用总共380份牛血清，将B/ELISA的灵敏度与病毒中和（VN）试验进行了比较。其中，118份血清来自已知无牛流行热的地区，181份来自自然感染和实验感染BEFV的牛，33份是从分离出贝里马病毒（BERV）的哨兵公牛采集的连续血清样本，9份是从分离出金伯利病毒（KIMV）的哨兵母牛采集的血清，还有一组39份血清作为盲法试验样本。B/ELISA的总体结果与VN试验的结果相比良好。使用针对其他BEF血清群病毒（即KIM和BER病毒）的超免疫小鼠腹水进行试验，证明了该试验的单特异性，结果显示无明显交叉反应。与VN试验相比，该试验更简单、灵敏度更高，使其成为诊断和监测临床牛流行热的首选试验。
